ynamic Phosphorylation of CENP-A at Ser68 Orchestrates Its Cell-Cycle-Dependent Deposition at Centromeres
he H3 histone variant CENP-A is an epigenetic marker critical for the centromere identity and function. However, the precise regulation of the spatiotemporal deposition and propagation of CENP-A at centromeres during the cell cycle is still poorly understood. Here, we show that CENP-A is phosphorylated at Ser68 during early mitosis by Cdk1. Our results demonstrate that phosphorylation of Ser68 eliminates the binding of CENP-A to the assembly factor HJURP, thus preventing the premature loading of CENP-A to the centromere prior to mitotic exit. Because Cdk1 activity is at its minimum at the mitotic exit, the ratio of Cdk1/PP1 alpha activity changes in favor of Ser68 dephosphorylation, thus making CENP-A available for centromeric deposition by HJURP. Thus, we reveal that dynamic phosphorylation of CENP-A Ser68 orchestrates the spatiotemporal assembly of newly synthesized CENP-A at active centromeres during the cell cycle.
he H3 histone variant CENP-A is an epigenetic marker critical for the centromere identity and function. However, the precise regulation of the spatiotemporal deposition and propagation of CENP-A at centromeres during the cell cycle is still poorly understood. Here, we show that CENP-A is phosphorylated at Ser68 during early mitosis by Cdk1. Our results demonstrate that phosphorylation of Ser68 eliminates the binding of CENP-A to the assembly factor HJURP, thus preventing the premature loading of CENP-A to the centromere prior to mitotic exit. Because Cdk1 activity is at its minimum at the mitotic exit, the ratio of Cdk1/PP1 alpha activity changes in favor of Ser68 dephosphorylation, thus making CENP-A available for centromeric deposition by HJURP. Thus, we reveal that dynamic phosphorylation of CENP-A Ser68 orchestrates the spatiotemporal assembly of newly synthesized CENP-A at active centromeres during the cell cycle.
Xu, Rui-Ming、Li, Shangze、Hu, Hao、Shen, Jing、Lou, Jizhong、Yu, Zhouliang、Hu, Hao、Yang, Na、Zhang, Xiaodong、Chen, Ping、Dong, Shuo、Fang, Junnan、Wang, Zichen、Zhai, Linhui、Cui, Lei、Peng, Shengyi、Li, Guohong、Xu, Ping、Deng, Wenqiang、Fang, Junnan、Yu, Zhouliang、Peng, Shengyi、Cui, Lei、Wong, Jiemin、Wong, Jiemin、Zhou, Xiang、Wang, Wenjing、Deng, Wenqiang、Ou, Guangshuo、Yuan, Zengqiang
细胞生物学分子生物学遗传学
HROMATIN REQUIRESHAPERONE SCM3NA-SEQUENCESHJURPPROTEINRECOGNITIONOMAINKINETOCHORENUCLEOSOMESPROPAGATION
Xu, Rui-Ming,Li, Shangze,Hu, Hao,Shen, Jing,Lou, Jizhong,Yu, Zhouliang,Hu, Hao,Yang, Na,Zhang, Xiaodong,Chen, Ping,Dong, Shuo,Fang, Junnan,Wang, Zichen,Zhai, Linhui,Cui, Lei,Peng, Shengyi,Li, Guohong,Xu, Ping,Deng, Wenqiang,Fang, Junnan,Yu, Zhouliang,Peng, Shengyi,Cui, Lei,Wong, Jiemin,Wong, Jiemin,Zhou, Xiang,Wang, Wenjing,Deng, Wenqiang,Ou, Guangshuo,Yuan, Zengqiang.ynamic Phosphorylation of CENP-A at Ser68 Orchestrates Its Cell-Cycle-Dependent Deposition at Centromeres[EB/OL].(2016-05-12)[2025-06-06].https://chinaxiv.org/abs/201605.01469.点此复制
评论