减毒鼠伤寒沙门氏菌调节肿瘤微环境代谢在肿瘤治疗中的应用研究

Objective: the incidence of pancreatic cancer is increasing year by year in the world, and the 5-year survival rate is less than 10%. Pancreatic cancer is a kind of"Cold" tumor with poor infiltration of immune cells. Immunotherapy has no significant effect on it, so it is urgent to explore a new therapy for pancreatic cancer. In this study, an engineered strain SLpCGL(plus) encoding cysteine hydrolase (CGL) was designed using attenuated Salmonella enterica ppGpp as a vector for the treatment of pancreatic cancer. Methods: the engineered strain SLpCGL(plus) was constructed with pBAD induced expression system. In vitro experiments, the inhibitory effect of SLpCGL(plus) on pancreatic cancer in vitro was examined using crystal violet staining.Results: SLpCGL(plus) significantly inhibited the growth of pancreatic cancer. Conclusion: In this study, we successfully developed a Salmonella enterica method to inhibit pancreatic cancer tumor growth by targeting cysteine metabolism in the tumor microenvironment. It provides a new idea for the clinical treatment of pancreatic cancer.