改良法制备富血小板血浆促进体外原代培养成骨细胞增殖的实验研究
HE EFFECT OF PLATELET-RICH PLASMA ON PROLIFERATION OF RAT AND HUMAN OSTEOBLAST IN VITRO
本研究通过富血小板血浆（platelet-rich plasma, PRP）来源的富生长因子血清(serum rich in growth factors, SRGF)作用于体外培养的大鼠成骨细胞和人成骨细胞探讨对体外原代培养成骨细胞增殖的促进作用。结果发现改良法制备的PRP来源的SRGF含有高浓度的TGF-β1和PDGF-AB，具有促进成骨细胞增殖的作用。
o study the effect of serum rich in platlelet-released growth factors (SRGF) on the biological functions of human and rat osteoblast. Human osteoblast were treated with 5% human-SRGF , and rat osteoblast were treated with 5% rat-SRGF. Cellular mitogenic activity was evaluated by thiazoly blue (MTT) colorimetric assay. The result showed that SRGF can up-regulating the proliferation of osteoblast.
宋扬、宫苹、巢永烈
基础医学生物科学研究方法、生物科学研究技术生理学
富血小板血浆 转化生长因子 血小板源性生长因子 成骨细胞
platelet-rich plasma， osteoblast， transforming growth factors， platelet-derived growth factors
宋扬,宫苹,巢永烈.改良法制备富血小板血浆促进体外原代培养成骨细胞增殖的实验研究[EB/OL].(2005-01-24)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/200501-85.点此复制
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