基于重金属离子(Hg2+)对碳纳米颗粒猝灭效应的适配体核酸探针检测ATP的研究
n aptasensor for ATP detection based on the quenching effect of Hg2+ on nano carbon dots
三磷酸腺苷（adenosine triphosphate，ATP）是一类普遍存在的能源物质，它广泛存在于各种生物体内，并发挥了极其重要的作用。通过对ATP的含量进行检测，可以对药物或生物制剂对细胞的杀伤作用进行客观评价，并且，可以对一些疾病进行早期的诊断及治疗。本试验通过水热法制备得到颜色均一、性能稳定的纳米碳点。基于重金属离子(Hg2+)对碳点的猝灭效应，构建了一个新型核酸适配体生物传感器对ATP进行快速检测。
denosine triphosphate (ATP) is an ubiquitous energy substance, it presented widely in all kinds of organisms and played a very important role. The killing effect of drugs or biological agents on cells can be objectively evaluated via detect the content of ATP, moreover, early diagnosis and treatment of certain disease can be achieved. In this research, nano carbon dots with uniform color and stable properties were obtained by hydrothermal method, an original aptasensor for rapid detection of ATP was constructed based on the quenching effect of Hg2+ on carbon nanoparticle.
李众杰、刘柯驿、邓乐
生物科学研究方法、生物科学研究技术生物化学生物物理学
三磷酸腺苷，碳点，汞离子，适配体，生物传感器
denosine triphosphate nano carbon dots Hg2+ aptamer biosensor
李众杰,刘柯驿,邓乐.基于重金属离子(Hg2+)对碳纳米颗粒猝灭效应的适配体核酸探针检测ATP的研究[EB/OL].(2016-01-11)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201601-200.点此复制
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