转Mfn1基因牛体细胞克隆胚胎的发育

Mitofusin 1 is critical to keep the frequent and continual cycles of mitochondrial fusion and fission, and regulate mitochondrial morphogenesis and complementation between mitochondrial genomes in eukaryotic cells. The heteroplasmic mitochondria existing in somatic cell cloned embryos is detrimental to the embryonic development. In the study, mfn1 gene was cloned by transgenic technique and ligated into the pEGFP-C1 vector. The construsted vector pEGFP-mfn was transfected into cow fetal fibroblasts, and then cell clones stably expressing GFP were screened.Somatic cell nuclear transfer embryos were obtained using the cells transfected Mfn1 as donor nucleus, and cow oocytes as ricipient cytoplasm.The results indicated that the expression levels of Mfn1 in the transgenic embryos were significantly higher than those in the control embryos, the development rates of transgenic embryos were similar to those of control embryos from 2-cell to 8-cell stages,but from 8-cell stage onwards, they were significantly higher in transgenic embryos than those in control embryos. The enhanced expression of Mfn1 may alleviate the side effects caused by the heteroplasmic mitochondria in somatic cell nuclear transfer embryos,and improve the development rate.