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砷(As)对凤尾蕨属植物体内黄酮类物质的影响

Effect of arsenic on flavonoid contents in Pteris species

中文摘要英文摘要

本实验在不同As浓度条件下处理2种砷超富集植物井栏边草(Pteris multifida)、蜈蚣草(Pteris vittata)和1种非砷超富集植物半边旗(Pteris semipinnata),采用高效液相色谱法(HPLC)测定植物体内5种类黄酮含量。另外,研究了在两个As处理浓度(0、20 mg As L-1)下井栏边草和半边旗体内黄酮类物质的时间诱导效应。结果表明:在相同As浓度处理条件下,叶片中槲皮素、异槲皮甙和山奈酚的含量不存在显著差;根部芸香苷、槲皮素、山奈酚和总黄酮含量也不存在显著差异。时间诱导效应研究表明:在20 mg L-1As处理条件下植物中芸香苷、金丝桃甙、槲皮素、山奈酚和总黄酮含量存在显著性差异。结果表明,砷超富集植物体内黄酮类物质的变化与其对砷富集特性无直接关联。

Five flavonoids were determined in twco arsenic (As) hyperaccumulators (Pteris multifida and Pteris vittata) and a non-hyperaccumulator (Pteris semipinnata) from uncontaminated and As contaminated sites exposed to different As concentrations under hydroponic conditions by high-performance liquid chromatography (HPLC). The time-course effect of flavonoid induction in P. multifida and P. semipinnata were also studied under 0 and 20 mg As L-1 treatments at different time. There were no significant differences in contents of quercetin, isoquercitrin and kaempferol in the fronds, and also no significant differences in contents of rutin, quercetin, kaempferol and total flavonoids in the roots of the three plants studied under the same As treatment. As for time-course effect, significant differences were observed in contents of rutin, quercetin, hyperin, kaempferol and total flavonoids among times at 20 mg As L-1 treatment. In general, the changes of flavonoid contents in As hyperaccumulators did not directly contribute to As accumulation.

王宏镔、叶志鸿

植物学环境生物学生物科学现状、生物科学发展

黄酮类物质超富集植物高效液相色谱

FlavonoidsFernrsenicHyperaccumulatorsHPLC

王宏镔,叶志鸿.砷(As)对凤尾蕨属植物体内黄酮类物质的影响[EB/OL].(2010-02-03)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201002-150.点此复制

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