常用分子检测技术在儿童龋病微生物多样性研究中的应用

ental caries is one of the most common chronic infectious diseases worldwide. And causes increasing harm to children's physical and mental health. Microorganisms are one of the main factors that cause dental caries. The types, cariogenic mechanisms and diversity of cariogenic microorganisms are hot topics in oral microbiology research. Modern molecular biological techniques, molecular biological detection techniques are now widely used in microbiological research. These early detection and prevention methods play an important role in reducing the incidence of caries disease in children. This review focused on the research advancement of some modern molecular biotechnology in oral microbial diversity research, including16S rDNA clone library, gene chip, polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), restriction fragment length polymorphism (RFLP), real-time polymerase chain reaction (RT-PCR), and pyrosequencing platform as the representative of the second High-throughput sequencing technology. In additions, it also made a future prospect for the study of oral microbial diversity in children.