基于盐酸羟胺合成的金纳米颗粒对深红红螺菌表面修饰的方法

We explored the surface modification of live R.rubrum through direct deposition of gold nanoparticles.The gold nanoparticles were prepared from hydroxylamine hydrochloride (HAHC). By using the direct deposition method, we successfully modified R.rubrum with gold nanoparticle with high efficicy through hydrogen bonding betweenprotein or lipid polysaccharides amine groups on bacterial surfaceand surface hydroxyl groups on gold nanoparticle. The pH value of the reaction system played a vital role on the direct deposition of gold nanoparticles on bacteria with the optimized pH value of 3. Under pH 3, the R.rubrum cells were homogeneously covered with a large number of gold nanoparticles with the spiral morphology well preserved. The direct deposition method using HAHC-gold nanoparticles was generalizable, which was successfully applied to for both Gram-negative and Gram-positive bacteria.