羽状鸡冠花幼苗玻璃花超低温保存研究

ryopreservation is considered to be the best way for long-term germplasm conservation. As few reports about seedling cryopreservation, a procedure has been developed for vitrification of Celosia plumosa ‘Fresh Look’seedlings. The procedure was: seedlings with 2-3 mm radicle length were loaded at 25℃ for 40min, then dehydrated seedlings in vitrification solution (PVS2) at 0℃for 60 min, immersed seedlings into LN2 directly and thawing in a water bath at 40℃ for 60s, following that, washed twice (10min each) with unloading solution added with 400 U?mL-1 CAT. Then recultured vitrified seedlings on MS medium in darkness for 7ds prior to exposure to the light. The regeneration rate reached 83.33±1.57%.