乙醇－黄原胶耦合发酵系统的初步研究

he feasibility and technology of the ethanol-Xanthan coupled two-stage fermentation was investigated in this work. When ethanol fermentation was stopped ahead of time and the solid content was removed by centrifugation, the ethanol in the broth was recovered by distillation and the resulting thin distiller with a lot of nutrients was employed for Xanthan fermentation. Test in flask culture and 7L batch fermenter proved the feasibility of this novel technology. The optimal condition for this coupled fermentation was as follows: the thin distiller of ethanol fermentation (60h) using cassava starch was chosen for Xanthan fermentation, 2 g/L of potassium dihydrogen phosphate was added in the medium and inorganic nitrogen source was not required. Under the above condition, 20 g/L Xanthan was obtained in 7L fermenter with a yield of 50%.