国家预印本平台

生物技术

生物技术通过改造生物系统解决健康、农业与环境问题。CRISPR-Cas9基因编辑、合成生物学、器官芯片与类器官培养是核心方向。研究趋势包括mRNA疫苗技术、微生物组工程、AI驱动的蛋白质设计，以及生物制造在碳中和中的潜力。

作者：Lin Lin；Devitt M?ller Henrik；Xu Xun；Regenberg Birgitte；Luo Yonglun；Liu Xin；Xiang Xi；Petersen Trine Skov；Zhang Xiuqing；Jensen Uffe Birk；Yang Luhan；Church George M.；Wang Jian；Bolund Lars；Huang Jinrong；Yang Huanming；Kjeldsen Eigil

发表日期：2025-03-28

Endogenous CRISPR arrays for scalable whole organism lineage tracing

发表日期：2025-03-28

CRISPR-Cas9-mediated Large Cluster Deletion and Multiplex Genome Editing in Paenibacillus polymyxa

作者：Teckentrup Christa；Schmid Jochen；Meliawati Meliawati

发表日期：2024-09-09

CRISPR-Cas9-assisted native end-joining editing offers a simple strategy for efficient genetic engineering in Escherichia coli

作者：Wang Jialei；Zhang Xueli；Huo Yi-Xin；Wang Xueqin；Ding Tingting；Huang Chaoyong；Wang Jingge；Bi Changhao；Ma Xiaoyan；Zhu Lin

发表日期：2024-09-09

Autonomous Remotely Controlled Closed System Transgenic Cell Technologies Robot: CRISPR.BOT

作者：Yelgen Gamze；Gelsin Beste；Cavrar Ilayda；Bal Enes；Teymur Tarik；Dogdu Solen；Araz Hasret；Erkek Fatmanur；Sert Berranur；Ay Yasin；Yilmaz Menekse Cagla；Baran Buse；Tiryaki Nulifer Neslihan；Tastan Cihan；Gulden Gamze；Odabas Sibel Pinar

发表日期：2024-09-09

Functional diversification of hybridoma produced antibodies by CRISPR/HDR genomic engineering

作者：Becker Anouk M.D.；Dalen Duco van；van Bruggen J. Armando C.；Bentlage Arthur E.H.；Heck Albert J.R.；Scheeren Ferenc A.；Nederend Maaike；Hagemans Iris M.；Fransen Marieke F.；Vidarsson Gestur；der Schoot Johan M.S. van；Dolen Yusuf；Valente Michael；Cevirgel Alper；Le Gall Camille M.；Figdor Carl G.；Leusen Jeanette H.W.；Caval Tomislav；Verdoes Martijn；Engelfriet M；Fennemann Felix L.

发表日期：2025-03-28

Na?ve and in vitro -activated primary mouse CD8 + T cells retain in vivo immune responsiveness after electroporation-based CRISPR/Cas9 genetic engineering

作者：Yerly Laura；Abe Jun；Pfenninger Petra

发表日期：2025-03-28

A small PAM optimises target recognition in the CRISPR-Cas immune system

作者：Melia E. Bonomo

发表日期：2024-09-09

CRISPR Mediated Transactivation in the Human Disease Vector Aedes aegypti

作者：Dimopoulos George；Akbari Omar；Dong Yuemei；Yang Ting；Li Ming；James Anthony A.；Benetta Elena Dalla；Bui Michelle；Buchman Anna；Zhao Yunchong；Perry Michael W.；Antoshechkin Igor A；Bottino-Rojas Vanessa

发表日期：2025-03-28

Detailed mechanisms for unintended large DNA deletions with CRISPR, base editors, and prime editors

作者：Oh Minsik；Kim Segi；Habib Omer；Kim Chan Hyuk；Kim Sun；Kim Seok-Hoon；Hwang Gue-ho；Bae Sangsu；Jang Hyeon-Ki；Kim Heon Seok

发表日期：2025-03-28